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[Objective]Study was carried out to study the low-phosphorus tolerance characteristics of different soybean varieties during the seedling stage, clarify the evaluation indicators for identifying low-phosphorus tolerance in soybean seedlings under lowphosphorus stress, and screen out low-phosphorus-tolerant varieties. [Method]Using 318 soybean varieties as the experimental materials, low-phosphorus stress treatment(LP, 0.05 mmol·L-1KH2 PO4) and normal phosphorus treatment(NP, 0.5 mmol·L-1KH2 PO4)were set up at the seedling stage. Nine indicators including plant height, main root length, above-ground dry weight, root dry weight,total root length, root surface area, root volume, root average diameter, and root tip number were measured. The low-phosphorus tolerance coefficients of each indicator were calculated. Variability analysis, correlation analysis, principal component analysis, and cluster analysis were conducted to analyze the characteristics of the indicators. The membership function method was combined to comprehensively evaluate the low-phosphorus-related indicators and low-phosphorus tolerance of different soybean varieties. [Result]There were extremely significant differences among the varieties for all 9 indicators. Except for the main root length and root tip number, there were significant differences in the other 7 indicators between the phosphorus treatments. The low-phosphorus tolerance coefficients of the 9 indicators could be simplified to 5 principal components. The cumulative contribution rate in the LP treatment reached 88.07%. A regression equation for evaluating the low-phosphorus tolerance characteristics of soybean seedlings was established: D =-0.454 + 0.221X_1+ 0.320X_2+ 0.247X_5+ 0.073X_8+ 0.058X9, R2= 0.998(P < 0.01). The first-level evaluation indicators under the LP treatment were X1(plant height), X2(main root length), X5(total root length), X8(root average diameter), and X9(root tip number). Among the 318 soybean materials, 61 materials belonged to low-phosphorus sensitive types, 102 materials belonged to low-phosphorus moderately sensitive types, 95 materials belonged to moderately tolerant types, and 60 materials belonged to tolerant types. [Conclusion]Five low-phosphorus tolerance indicators at soybean seedling stage, namely plant height, main root length,total root length, root average diameter, and root tip number were selected. 60 soybean varieties were identified as low-phosphorustolerant. The research results provide scientific methods for the identification and selection of soybean low-phosphorus-tolerant varieties, and also provide theoretical basis and material foundation for the exploration of low-phosphorus tolerance functional genes in soybeans and efficient utilization of phosphorus nutrition.
[1]曹彦蕾.我国大豆产业发展和育种研究现状启示[J].黑龙江农业科学,2025(1):86-93.CAO Y L.Current situation of soybean industry development and breeding countermeasures in China[J].Heilongjiang Agricultural Sciences,2025(1):86-93.
[2]何德鑫,赵翔,张雪静,等.大豆连作根际土壤微生物群落构建特征[J].沈阳农业大学学报,2024,55(1):1-8.HE D X,ZHAO X,ZHANG X J,et al.Characteristics of microbial community construction in soybean continuous cropping rhizosphere soil[J].Journal of Shenyang Agricultural University,2024,55(1):1-8.
[3]孙畅,刘美玲,刘子君,等.低磷胁迫下大豆苗期根系性状全基因组关联分析[J].沈阳农业大学学报,2024,55(6):663-676.SUN C,LIU M L,LIU Z J,et al.Genome-wide association analysis of root traits at seedlings stage under low phosphorus stress in soybean(Glycine max L.)[J].Journal of Shenyang Agricultural University,2024,55(6):663-676.
[4]刘海旭,吴俊江,王金生,等.大豆耐低磷研究进展[J].大豆科学,2017,36(4):639-644.LIU H X,WU J J,WANG J S,et al.Progress of research on tolerance to low-phosphorus stress in soybean[J].Soybean Science,2017,36(4):639-644.
[5]马祥庆,梁霞.植物高效利用磷机制的研究进展[J].应用生态学报,2004,15(4):712-716.MA X Q,LIANG X.Research advances in mechanism of high phosphorus use efficiency of plants[J].Chinese Journal of Applied Ecology,2004,15(4):712-716.
[6]尹添情,刘鹏园,赵斌,等.玉米苗期低磷胁迫下根系性状特征及评价方法[J].河南科技学院学报(自然科学版),2025,53(6):11-20.YIN T Q,LIU P Y,ZHAO B,et al.Characteristics and evaluation methods of maize root characteristics under low phosphorus stress at seedling stage[J].Journal of Henan Institute of Science and Technology(Natural Sciences Edition),2025,53(6):11-20.
[7]TANG H L,CHEN X Y,GAO Y J,et al.Alteration in root morphological and physiological traits of two maize cultivars in response to phosphorus deficiency[J].Rhizosphere,2020,14:100201.
[8]葛家豪,雷欣月,王清明,等.苦荞苗期耐低磷种质筛选及耐低磷综合评价[J].作物学报,2025,51(11):2911-2922.GE J H,LEI X Y,WANG Q M,et al.Screening of low-phosphorus tolerant germplasm and comprehensive evaluation of low phosphorus tolerance in Tartary buckwheat at seedling stage[J].Acta Agronomica Sinica,2025,51(11):2911-2922.
[9]梁腾月,谷勇哲,马英杰,等.大豆耐低磷性全基因组关联分析[J].植物遗传资源学报,2023,24(1):237-251.LIANG T Y,GU Y Z,MA Y J,et al.Genome wide association study of low phosphorus tolerance in soybean(Glycine max L.)[J].Journal of Plant Genetic Resources,2023,24(1):237-251.
[10]GAO D D,SHENG R,WHITELEY A S,et al.Effect of phosphorus amendments on rice rhizospheric methanogens and methanotrophs in a phosphorus deficient soil[J].Geoderma,2020,368:114312.
[11]刘子君.低磷胁迫对大豆根系形态、光合特性及蔗糖代谢的影响[D].沈阳:沈阳农业大学,2025.LIU Z J.Effects of low phosphorus stress on root morphology,photosynthetic characteristics and sucrose metabolism of soybean[D].Shenyang:Shenyang Agricultural University,2025.
[12]刘渊,李喜焕,王瑞霞,等.大豆耐低磷指标筛选与耐低磷品种鉴定[J].中国农业科技导报,2015,17(4):30-41.LIU Y,LI X H,WANG R X,et al.Screen indexes for soybean tolerance to phosphorus deficiency and identification of low phosphorus tolerant soybean cultivars[J].Journal of Agricultural Science and Technology,2015,17(4):30-41.
[13]武海燕,李喜焕,李文龙,等.大豆耐低磷性状鉴定及优异种质筛选[J].河南农业科学,2020,49(1):61-67.WU H Y,LI X H,LI W L,et al.Identification of low phosphorus tolerant traits and selection of elite genotypes in soybean[J].Journal of Henan Agricultural Sciences,2020,49(1):61-67.
[14]刘露露,汪军成,姚立蓉,等.不同春小麦品种耐低磷性评价及种质筛选[J].中国生态农业学报(中英文),2020,28(7):999-1009.LIU L L,WANG J C,YAO L R,et al.Evaluation of low phosphorus tolerance and germplasm screening of spring wheat[J].Chinese Journal of Eco-Agriculture,2020,28(7):999-1009.
[15]熊楚雯,郭智滨,周强华,等.大豆转录因子NAC1耐低磷胁迫的功能研究[J].中国农业科学,2024,57(3):442-453.XIONG C W,GUO Z B,ZHOU Q H,et al.Function analysis of the soybean transcription factor NAC1 in tolerance to low phosphorus[J].Scientia Agricultura Sinica,2024,57(3):442-453.
[16]王辉.磷高效大豆品种的筛选及苗期鉴定方法[D].沈阳:沈阳农业大学,2020.WANG H.Screening and seedling identification of soybean varieties with high phosphorus efficiency[D].Shenyang:Shenyang Agricultural University,2020.
[17]吴瑶瑶,贺金萍,薛承康,等.甘薯耐盐性综合评价及耐盐指标筛选[J].南京农业大学学报,2025,48(3):572-580.WU Y Y,HE J P,XUE C K,et al.Comprehensive evaluation of salt tolerance in sweetpotato and screening of salt tolerance parameters[J].Journal of Nanjing Agricultural University,2025,48(3):572-580.
[18]付连双,和阳升,王欣晨,等.179份高粱种质资源表型鉴定与遗传多样性分析[J].东北农业大学学报,2024,55(2):1-9.FU L S,HE Y S,WANG X C,et al.Phenotypic identification and genetic diversity analysis of 179 sorghum germplasm resources[J].Journal of Northeast Agricultural University,2024,55(2):1-9.
[19]黄俊霞,冀宝璐,赵伟,等.大豆种质鼓粒期源库关系及籽粒皱缩性鉴定指标筛选[J].沈阳农业大学学报,2025,56(3):14-22.HUANG J X,JI B L,ZHAO W,et al.Source-sink relationship of soybean germplasm at bulging stage and screening of identification indexes of grain wrinkles[J].Journal of Shenyang Agricultural University,2025,56(3):14-22.
[20]苑乂川,陈小雨,李明明,等.谷子苗期耐低磷种质筛选及其根系保护酶系统对低磷胁迫的响应[J].作物学报,2019,45(4):601-612.YUAN(A/Y)C,CHEN X Y,LI M M,et al.Screening of germplasm tolerant to low phosphorus of seedling stage and response of root protective enzymes to low phosphorus in foxtail millet[J].Acta Agronomica Sinica,2019,45(4):601-612.
[21]侯莹莹,胡小梅.3株玉米根际解磷菌筛选鉴定及促生作用研究[J].东北农业大学学报,2024,55(5):46-55.HOU Y Y,HU X M.Screening and identification of three strains of phosphate-solubilizing bacteria from maize rhizosphere and their roles in plant growth promoting effect[J].Journal of Northeast Agricultural University,2024,55(5):46-55.
[22]丁广大,陈水森,石磊,等.植物耐低磷胁迫的遗传调控机理研究进展[J].植物营养与肥料学报,2013,19(3):733-744.DING G D,CHEN S S,SHI L,et al.Advances in genetic regulation mechanism of plant tolerance to phosphorus deficiency[J].Journal of Plant Nutrition and Fertilizer,2013,19(3):733-744.
[23]王英,李喜焕,张彩英.河北大豆地方品种耐低磷种质筛选[J].大豆科学,2009,28(4):588-594.WANG Y,LI X H,ZHANG C Y.Screening of low-P tolerant soybean landraces from Hebei growing-areas[J].Soybean Science,2009,28(4):588-594.
[24]LIANG C Y,PINEROS M A,TIAN J,et al.Low pH,aluminum,and phosphorus coordinately regulate malate exudation through GmALMT1 to improve soybean adaptation to acid soils[J].Plant Physiology,2013,161(3):1347-1361.
[25]LYNCH J P.Rightsizing root phenotypes for drought resistance[J].Journal of Experimental Botany,2018,69(13):3279-3292.
[26]丁玉川,陈明昌,程滨,等.不同大豆品种磷吸收利用特性比较研究[J].西北植物学报,2005,25(9):1791-1797.DING Y C,CHEN M C,CHENG B,et al.Phosphorous uptakes and uses of different soybean varieties[J].Acta Botanica Boreali-Occidentalia Sinica,2005,25(9):1791-1797.
[27]赵艳红,陈怀珠,杨守臻,等.大豆耐低磷鉴定指标研究[J].大豆科学,2009,28(1):175-177.ZHAO Y H,CHEN H Z,YANG S Z,et al.Identification indexes of the low-phosphorus tolerance of soybean[J].Soybean Science,2009,28(1):175-177.
[28]WANG X X,LI H B,CHU Q,et al.Mycorrhizal impacts on root trait plasticity of six maize varieties along a phosphorus supply gradient[J].Plant and Soil,2020,448(1):71-86.
[29]王金生,王君,吴俊江,等.基于GGE-biplot的大豆耐低磷资源筛选[J].大豆科学,2018,37(4):511-516.WANG J S,WANG J,WU J J,et al.Screening of soybean germplasm tolerant to low phosphorus based on GGE-biplot[J].Soybean Science,2018,37(4):511-516.
[30]曾维英,孙祖东,蔡昭艳,等.不同大豆新品种苗期耐低磷的鉴定与筛选[J].广西农学报,2015,30(1):14-17.ZENG W Y,SUN Z D,CAI Z Y,et al.The identification and selection of different new soybean varieties with high phosphorus efficiency in seedling stage[J].Journal of Guangxi Agriculture,2015,30(1):14-17.
[31]许仙菊,张永春.植物耐低磷胁迫的根系适应性机制研究进展[J].江苏农业学报,2018,34(6):1425-1429.XU X J,ZHANG Y C.Research progress on the root adaptation mechanism of plants under low phosphorus stress[J].Jiangsu Journal of Agricultural Sciences,2018,34(6):1425-1429.
[32]李月仙,段春芳,宋记明,等.30份木薯种质资源表型性状遗传多样性分析及综合评价[J].植物遗传资源学报,2025,26(11):2164-2180.LI Y X,DUAN C F,SONG J M,et al.Genetic diversity analysis and comprehensive evaluation of phenotypic traits in 30 cassava germplasm resources[J].Journal of Plant Genetic Resources,2025,26(11):2164-2180.
[33]蒋昂辰,李琰,李雨晨,等.21份无芒雀麦种质农艺性状及种子产量的综合评价与优异种质筛选[J].作物学报,2025,51(11):2958-2970.JIANG A C,LI Y,LI Y C,et al.Comprehensive evaluation of agronomic traits and seed yield of 21 Bromus inermis germplasm and screening of superior germplasm[J].Acta Agronomica Sinica,2025,51(11):2958-2970.
[34]霍庆平,李建波,门秀丽,等.大麦苗期耐盐种质鉴定与评价指标筛选[J].沈阳农业大学学报,2022,53(6):665-676.HUO Q P,LI J B,MEN X L,et al.Identification of elite salt-tolerant barley germplasms at the seedling stage and screening for effective evaluation indexes[J].Journal of Shenyang Agricultural University,2022,53(6):665-676.
[35]王晶琴,石贵阳,杨松花,等.不同基因型大豆苗期耐低磷性鉴定及指标筛选[J].福建农业学报,2022,37(6):702-711.WANG J Q,SHI G Y,YANG S H,et al.Determination and index selection on tolerance of soybean seedlings to phosphorus deficiency in soil[J].Fujian Journal of Agricultural Sciences,2022,37(6):702-711.
[36]周秀文,张晓蕊,孙贺祥,等.大豆种质萌发期和苗期耐盐性评价[J].沈阳农业大学学报,2022,53(3):257-264.ZHOU X W,ZHANG X R,SUN H X,et al.Evaluation of salt tolerance of soybean germplasms at germination and seedling stages[J].Journal of Shenyang Agricultural University,2022,53(3):257-264.
[37]张楠,张鹤,白百一,等.不同耐受型花生品种对盐胁迫的生理响应及其产量差异[J].沈阳农业大学学报,2024,55(2):129-137.ZHANG N,ZHANG H,BAI B Y,et al.Physiological responses and yield differences of different tolerant peanut cultivars to salt stress[J].Journal of Shenyang Agricultural University,2024,55(2):129-137.
[38]周思远,毕惠惠,程西永,等.小麦耐低磷相关性状的全基因组关联分析[J].植物遗传资源学报,2020,21(2):431-445.ZHOU S Y,BI H H,CHENG X Y,et al.Genome-wide association study of low-phosphorus tolerance related traits in wheat[J].Journal of Plant Genetic Resources,2020,21(2):431-445.
[39]米热扎提江·喀由木,西尔艾力·吾麦尔江,李晓曈,等.棉花苗期耐低磷种质筛选及耐低磷综合评价[J].中国农业科学,2023,56(21):4150-4162.KAYOUMU M R Z T J,WUMAIERJIANG X E A L,LI X T,et al.Screening of low phosphorus tolerant germplasm in cotton at seedling stage and comprehensive evaluation of low phosphorus tolerance[J].Scientia Agricultura Sinica,2023,56(21):4150-4162.
[40]栗振义,张绮芯,仝宗永,等.不同紫花苜蓿品种对低磷环境的形态与生理响应分析[J].中国农业科学,2017,50(20):3898-3907.LI Z Y,ZHANG Q X,TONG Z Y,et al.Analysis of morphological and physiological responses to low pi stress in different alfalfas[J].Scientia Agricultura Sinica,2017,50(20):3898-3907.
[41]刘灵,廖红,王秀荣,等.不同根构型大豆对低磷的适应性变化及其与磷效率的关系[J].中国农业科学,2008,41(4):1089-1099.LIU L,LIAO H,WANG X R,et al.Adaptive changes of soybean genotypes with different root architectures to low phosphorus availability as related to phosphorus efficiency[J].Scientia Agricultura Sinica,2008,41(4):1089-1099.
[42]ZHOU X Y,TIAN Y M,QU Z P,et al.Comparing the salt tolerance of different spring soybean varieties at the germination stage[J].Plants,2023,12(15):2789.
[43]杨春婷,张永清,马星星,等.苦荞耐低磷基因型筛选及评价指标的鉴定[J].应用生态学报,2018,29(9):2997-3007.YANG C T,ZHANG Y Q,MA X X,et al.Screening genotypes and identifying indicators of different Fagopyrum tataricum varieties with low phosphorus tolerance[J].Chinese Journal of Applied Ecology,2018,29(9):2997-3007.
[44]刘允熙,罗佳佳,雷健,等.柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J].草地学报,2021,29(5):876-883.LIU Y X,LUO J J,LEI J,et al.Screening of phosphorus efficiency germplasm and analysis of root morphology responding to phosphorus deficiency in Stylosanthes guianensis[J].Acta Agrestia Sinica,2021,29(5):876-883.
[45]朱瑞利,毛琳琳,王龙,等.苗期耐低磷毛叶苕子品种的筛选及其磷效率类型评价[J].中国土壤与肥料,2022(3):148-157.ZHU R L,MAO L L,WANG L,et al.Screening of Vicia villosa varieties with low phosphorus tolerance at seedling stage and evaluation of their phosphorus efficiency types[J].Soil and Fertilizer Sciences in China,2022(3):148-157.
[46]任瑞玉,李亚伟,董孔军,等.谷子种质资源农艺性状与品质的多样性分析及综合评价[J].干旱地区农业研究,2025,43(5):1-14.REN R Y,LI Y W,DONG K J,et al.Diversity analysis and comprehensive evaluation of agronomic traits and quality of foxtail millet germplasm resources[J].Agricultural Research in the Arid Areas,2025,43(5):1-14.
Basic Information:
China Classification Code:S565.1
Citation Information:
[1]JIA Sen,ZHAO Xiang,HUANG Junxia ,et al.Screening of Low-Phosphorus Tolerance Indicators at Seedling Stage and Identification of Low-Phosphorus-Tolerant Varieties of Soybean[J].Journal of Shenyang Agricultural University,2026,57(02):9-20.
Fund Information:
黑龙江省自然科学基金资助项目（PL2025C058）
2026-03-23
2026-03-23
2026-03-23