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[Objective]Study was carried out to investigate the function of the aluminum tolerance-related gene OsAlR2 under aluminum stress, and to elucidate the regulatory mechanism by which OsAlR2 participates in regulating aluminum stress responses.[Methods]In our previous study, an aluminum tolerance-related gene OsAlR2 was identified. The knockout mutant ko-alr2 and overexpression mutant oe-alr2 were generated, and the phenotypes and physiological mechanisms of these lines were analyzed.[Results]After aluminum treatment, the root length and root activity of oe-alr2 were lower than those of the wild type, whereas no significant differences were observed between ko-alr2 and the wild type. In terms of aluminum content, the Al3+ content in ko-alr2 was significantly lower than that in the wild type, while no obvious difference was found in oe-alr2 compared with the wild type.Subsequent section observation showed that root cells of oe-alr2 exhibited varying degrees of damage after aluminum treatment,whereas no obvious damage was detected in the wild type and ko-alr2. Under aluminum stress, the MDA content in oe-alr2 was significantly higher than that in the wild type, while the MDA content in ko-alr2 was markedly lower. Assays of the reactive oxygen species-scavenging enzymes CAT and SOD revealed that the activities of CAT and SOD in ko-alr2 were significantly higher than those in the wild type, whereas the activities in oe-alr2 were lower. The expression levels of several known aluminum tolerance genes,including OsFRDL2, OsSTAR1, and OsCDT3, were determined. Under aluminum stress, the transcript levels of OsSTAR1 and OsCDT3 in ko-alr2 were significantly upregulated and higher than those in the wild type, while the opposite pattern was observed in oe-alr2.[Conclusion]These results demonstrate that the OsAlR2 gene is involved in regulating the defense of rice against aluminum toxicity. It negatively regulates aluminum tolerance in rice by modulating reactive oxygen species-scavenging capacity and the expression of aluminum tolerance-related genes. This study lays a foundation for further revealing the regulatory mechanism of this gene in response to aluminum stress at the rice seedling stage.
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Basic Information:
China Classification Code:S511
Citation Information:
[1]JIANG Sixü,XIE Hongyang,FENG Jing ,et al.Functional Analysis of OsAlR2 in Regulating Aluminum Tolerance in Rice[J].Journal of Shenyang Agricultural University,2026,57(04):12-19.
Fund Information:
国家重点研发计划项目（2024YFD1501505）; 辽宁省科技重大专项（2024JH1/11700006-3）
2026-07-28
2026-07-28
2026-07-28