LU Xiaochun1
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[Objective]Genotypic identification is a prerequisite for efficient breeding, and there is an urgent demand for earlymaturing and dwarf varieties in the sorghum-producing regions of Heilongjiang Province. This study aimed to establish a sorghum genotyping system based on functional markers and InDel markers to provide technical support for molecular marker-assisted breeding. [Methods]Using 24 sorghum backbone lines from Heilongjiang Province as validation materials, allelic variations were identified using molecular markers of 14 cloned functional genes. Meanwhile, the genome-wide detection system was developed based on the 35 pairs of InDel markers, which were developed based on resequencing data, as well as 40 pairs of SSR markers. [Results]Functional marker genotyping and InDel marker analysis revealed that the synergistic effect of dw3-sd7 with aa or dw1 significantly reduced plant height. All 24 lines carried mutations in the photoperiod-sensitive gene ma1, and additional mutations in ma5 and ma6 synergistically shortened the maturity period; however, some lines with the same functional genotype exhibited a difference of up to 26 days in maturity, suggesting the existence of unknown regulatory genes. The 35 pairs of InDel markers effectively distinguished sister lines with highly similar field phenotypes and compensated for gaps in marker coverage in certain chromosomal regions. [Conclusion]The combined functional marker and InDel marker system established in this study enables rapid genotyping of sorghum germplasm for traits such as plant height, maturity, and grain quality, providing a practical technical solution for parental selection, variety identification, and molecular marker-assisted breeding.
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Basic Information:
China Classification Code:S514
Citation Information:
[1]BAI Chunming,YAN Hongdong,CONG Ling ,et al.Development of a Multi-Trait Molecular Marker System and Its Application in Sorghum Breeding[J].Journal of Shenyang Agricultural University,2026,57(05):13-24.
Fund Information:
辽宁省种质创新藏粮于技专项计划项目（2023JH1/10200009,2023JH1/1020000903）; 辽宁省基础研究计划项目（2026JC4017）; 黑龙江省农业科学院农业科技创新跨越工程项目（CX23TS07）
2026-07-20
2026-07-20
2026-07-20